Save up to 20% on bulk orders · Free shipping over $200
Quick answer: A Certificate of Analysis (CoA) is the laboratory report for one specific batch, or lot, of a compound. To read a peptide CoA, confirm five things: the compound name, the lot number that matches your vial, the test date, the purity result from HPLC, and the molecular mass confirmed by mass spectrometry. If any of these are missing or do not match, the document does not verify your vial.
A Certificate of Analysis is a document issued by a testing laboratory that records the results of analytical tests performed on a single production lot. For research peptides it answers two questions every lab needs answered before an experiment: is this the right molecule, and how much of the material is that molecule rather than impurities.
A CoA only applies to the lot it names. A report for lot A tells you nothing about lot B, even when the compound name is identical. That is why reputable suppliers, including Pure Health Sciences, publish a separate CoA for every lot and print the lot number on the vial.
The header lists the compound name, the lot or batch number, the quantity tested and often the manufacture date. The lot number is the single most important field, because it is the link between the paper and the physical vial on your bench.
Look for the name of the laboratory that performed the analysis and the date of testing. Independent third-party testing carries more weight than an in-house report, because the lab has no stake in the result.
High-performance liquid chromatography separates the components of a sample. Purity is usually reported as the percentage of the total detected signal that belongs to the main peak. A result such as 99.2% means the target peptide accounts for 99.2% of the detected material. Many CoAs include the chromatogram itself: one tall, sharp peak with only small peaks around it is what you want to see.
Mass spectrometry measures the molecular mass of the material and compares it with the theoretical mass of the expected sequence. A close match confirms identity. Purity without identity is not enough: a sample can be very pure and still be the wrong compound.
Some reports add appearance (for example, white lyophilized powder), water content, counter-ion content or endotoxin results. These are useful extras, but identity and purity are the core.
CoA stands for Certificate of Analysis, a laboratory report that records test results for one specific lot of a compound.
Many research protocols call for 98% purity or higher. Pure Health Sciences only lists lots that test at 99% or higher by HPLC.
It is incomplete. HPLC measures purity but does not confirm identity, so a CoA should also include a mass spectrometry result that matches the expected molecular mass.
Each lot has its own Certificate of Analysis. Match the lot number on your vial to the report, or email info@purehealthsciences.com with your order and lot number and we will send it.
This article is for educational purposes for laboratory researchers. Pure Health Sciences products are sold for in vitro research use only and are not for human or veterinary use.
Every lot ships with its Certificate of Analysis. Free tracked shipping over $200 CAD.
Ordering research peptides in Canada? Use this seven-point checklist to judge supplier quality, documentation, shipping and research-use compliance before you buy.
HPLC measures how pure a peptide sample is, while mass spectrometry confirms what the molecule is. Here is how each test works and why a trustworthy CoA includes both.
Lyophilized peptides last longest sealed, frozen and dry. This lab guide covers storage temperatures, light and moisture control, and how to handle vials when you open them.
Pure Health Sciences supplies research compounds only to qualified researchers and laboratories. Please confirm before continuing.
Not a researcher? Leave this site. See our terms.